We then give an overview of new FCS modalities that are designed to cope with emerging challenges in intracellular applications, e.g. << 120 0 obj /Type /Pages Fig. << Much of the early modern work using this embryo model began with the papers of Kimmel.Several large laboratories in the US are now developing large breeding programs to carry out \"k… /Limits [(Section16) (maintitle)] 2014;1124:219–233. The ACQUIFER Imaging Machine is a versatile and flexible high content screening platform following a unique opto-mechanical design, optimized for non-adherent and motion-sensitive specimen. >> >> /D [29 0 R /FitR 307 413 545 413] /Contents 400 0 R Brain-wide neuronal dynamics during motor adaptation in zebrafish. /D [28 0 R /FitR 307 493 545 493] << /Annots [182 0 R 183 0 R 184 0 R 185 0 R 186 0 R 187 0 R 188 0 R 189 0 R 190 0 R 191 0 R /D [24 0 R /FitR 0 786 596 0] >> Figure 2. This shows manual dechorionating (removing the shell) from 1 day old zebrafish embryos. /D [29 0 R /FitR 307 174 545 174] /D [29 0 R /FitR 307 303 545 303] >> /Type /Page << Drug Discov. Commun.6, 7790 (2015). The zebrafish model is a powerful tool for studying developmental and apoptotic processes in vertebrates. /Creator (3B2 Total Publishing System 8.07e/W Unicode ) 122 0 obj In the last section we demonstrate … /Names 3 0 R /ModDate (D:20141113140224+01'00') << /D [21 0 R /FitR 307 682 545 682] endobj /Annots [283 0 R 284 0 R 285 0 R 286 0 R 287 0 R 288 0 R 289 0 R 290 0 R 291 0 R 292 0 R /D [27 0 R /FitR 307 543 545 543] 27 0 obj >> << /D [25 0 R /FitR 307 546 545 546] << (CR36) 60 0 R (CR37) 61 0 R (CR38) 62 0 R (CR39) 63 0 R (CR4) 64 0 R /Resources 281 0 R >> J Vis Exp. endobj /D [28 0 R /FitR 52 393 290 393] 16 0 obj /D [28 0 R /FitR 52 453 290 453] 351 0 R 352 0 R] /Metadata 2 0 R << >> << /Contents 353 0 R /D [28 0 R /FitR 52 104 290 104] (CR99) 129 0 R (HypASec1) 130 0 R (HypASec2) 131 0 R (HypASec3) 132 0 R (HypASec4) 133 0 R 2004;305:1007–1009. << /Count 1 /D [27 0 R /FitR 307 732 545 732] endobj >> /CropBox [0 -5.66927 595.276001 785.197021] endobj /EmbeddedFiles 8 0 R /Thumb 340 0 R Position of 10x images relative to 1 dpf zebrafish embryo. << /D [29 0 R /FitR 52 682 290 682] endobj >> /MediaBox [0 -5.66927 595.276001 785.197021] /D [29 0 R /FitR 307 632 545 632] Phage Therapy Application to Counteract Pseudomonas aeruginosa Infection in Cystic Fibrosis Zebrafish Embryos Authors: Cafora M, Forti F, Briani F, Ghisotti D, Pistocchi A endobj /D [27 0 R /FitR 307 492 545 492] These embryos have the great advantage that they develop as \"see through\" embryos, that is, all internal development can be clearly observed from the outside in the living embryo. 78 0 obj /MediaBox [0 -5.66927 595.276001 785.197021] Zebrafish or zebra danio (danio rerio) are seen as one of the latest \"models\" for vertebrate embryological development studies. /D [19 0 R /FitR 52 495 290 495] /D [19 0 R /FitR 52 334 290 334] /D [28 0 R /FitR 307 543 545 543] /ca 1 << >> 116 0 obj Low cost, easy handling, the requirement of small quantities of test compounds, and high t… << Solvent Violet 47 (SV47) and Disperse Blue 14 (DB14) are two anthraquinone dyes that were previously used in different formulations for the production of violet-colored smoke. >> /Thumb 355 0 R 150 0 obj << 106 0 obj /CropBox [0 -5.66927 595.276001 785.197021] Figure 2. endobj 58 0 obj >> (CR45) 70 0 R (CR46) 71 0 R (CR47) 72 0 R (CR48) 73 0 R (CR49) 74 0 R /D [20 0 R /FitR 307 246 545 246] >> >> The utility of fish embryos for pesticide hazard assessment was investigated by comparing published zebrafish embryo toxicity data from pesticides with median lethal concentration 50% (LC50) data for juveniles of 3 commonly tested fish species: rainbow trout, bluegill sunfish, and sheepshead minnow. /D [19 0 R /FitR 52 495 290 495] /Names [(CR67) 94 0 R (CR68) 95 0 R (CR69) 96 0 R (CR7) 97 0 R (CR70) 98 0 R endobj /Kids [10 0 R 11 0 R] We report the synthesis, characterization, and in vivo application of light-activated, negatively charged peptide nucleic acids (caged ncPNAs) for down-regulating gene expression during zebrafish embryonic development. << 224 0 R 225 0 R 226 0 R 227 0 R 228 0 R 229 0 R 230 0 R 231 0 R 232 0 R 233 0 R /Contents 203 0 R /Thumb 325 0 R << >> 8 0 obj ... Vitrification of zebrafish embryo blastomeres in microvolumes. /Title << endobj /D [28 0 R /FitR 307 134 545 134] >> 3. /Dest (Section17) /CropBox [0 -5.66927 595.276001 785.197021] << << << De Luca, E. et al. /CropBox [0 -5.66927 595.276001 785.197021] 44 0 obj 21138/ARC_/Arthritis Research UK/United Kingdom, MR/S00551X/1/MRC_/Medical Research Council/United Kingdom, 098411/Z/12/Z/WT_/Wellcome Trust/United Kingdom, 206444/Z/17/Z/WT_/Wellcome Trust/United Kingdom, Lam PY, Fischer RS, Shin WD, Waterman CM, Huttenlocher A. (Section12) 154 0 R (Section13) 155 0 R (Section14) 156 0 R (Section15) 157 0 R] /Contents [204 0 R 205 0 R 206 0 R 207 0 R 208 0 R 209 0 R 210 0 R 211 0 R] /Rotate 0 >> 192 0 R 193 0 R 194 0 R 195 0 R 196 0 R 197 0 R 198 0 R 199 0 R 200 0 R 201 0 R /Resources 354 0 R 1- Collect embryos at the one-cell stage in a 35 mm Petri dish with E3 medium (100 embryos per condition at least). 147 0 obj -. /Parent 10 0 R 274 0 R 275 0 R 276 0 R 277 0 R 278 0 R 279 0 R] << endobj endobj /Parent 10 0 R 103 0 obj endobj Mycobacterium marinum infection of adult zebrafish causes caseating granulomatous tuberculosis and is moderated by adaptive immunity. << >> /Limits [(CR1) (CR66)] /D [29 0 R /FitR 307 602 545 602] Zebrafish and humans share 70% of the same genes. << /Prev 172 0 R << 141 0 obj endobj endobj Position of 10x images relative to 1 dpf zebrafish embryo. >> >> 36 0 obj Here, we investigated the applicability of pollutant injection into the yolk of early zebrafish (Danio rerio) … << endobj /CropBox [0 -5.66927 595.276001 785.197021] << /D [28 0 R /FitR 52 194 290 194] << Zebrafish embryos provide a unique opportunity to visualize complex biological processes, yet conventional imaging modalities are unable to access intricate biomolecular information without compromising the integrity of the embryos. /Rotate 0 /TrimBox [0 -5.66927 595.276001 785.197021] Zebrafish embryos were kept in a 28 °C incubator in E3 media (5 mM NaCl, 0.33 mM MgSO 4, 0.33 mM CaCl 2, 0.17 mM KCl, and 0.1% methylene blue). << /Title () /D [29 0 R /FitR 52 194 290 194] endobj endobj endobj /Limits [(CR67) (Section15)] 52 0 obj /SA true 94 0 obj << Fig. /TrimBox [0 -5.66927 595.276001 785.197021] 37 0 obj doi: 10.1080/17460441.2017.1297416. endobj organization levels. During all the imaging processes of all the groups of fish, the /D [29 0 R /FitR 307 493 545 493] 12 0 obj /D [28 0 R /FitR 307 682 545 682] Zebrafish embryos were infected with P. aeruginosa to demonstrate that phage therapy is effective against P. aeruginosa infections as it reduces lethality, bacterial burden and pro-inflammatory immune response in CF embryos. Beacon is created at the end of the yolk sac so that the resulting image is set around the trunk of the embryo. Swaim LE, Connolly LE, Volkman HE, Humbert O, Born DE, Ramakrishnan L. Infect Immun. >> Early-life environmental exposure to toxic chemicals has a long-term influence on the development and health of living organisms. /Next 169 0 R Automated Microscopy for Zebrafish And Other Whole Organism Screening Applications. %PDF-1.3 /Last 17 0 R << /BM /Normal /D [27 0 R /FitR 307 513 545 513] /Contents [410 0 R 411 0 R] AU - Chen, Yongsheng. Zebrafish one to eight-cell embryos were microinjected with 6.5 ng VaDNA together with the firefly and Renilla reporter vectors as indicated in Legend to Figure 2 alone or in combination with 4 ng of the indicated morpholinos. << 146 0 obj /Type /Pages endobj >> /D [20 0 R /FitR 0 786 596 0] /Title endobj 121 0 obj & Huisken, J. Hyperspectral light sheet microscopy. << With this technique, parental transfer of lipophilic contaminants may be mimicked. << (CR27) 50 0 R (CR28) 51 0 R (CR29) 52 0 R (CR3) 53 0 R (CR30) 54 0 R /D [21 0 R /FitR 52 171 290 171] >> In zebrafish embryos, downstream gene expression can be greatly induced throughout the body by raising the temperature from 28.5°C to 38.0°C. pearing in the development of zebrafish, Danio (Brachydanio) rerio, embryos fertilized simultaneously in vitro (C. Walker and G. Streisinger, in Westerfield, 1994) and incubated at an optimal temperature with- out crowding (28.S0C, 5-10 embryos/ml). >> endobj >> /Type /Page 96 0 obj 145 0 obj 83 0 obj /D [27 0 R /FitR 307 582 545 582] << /Thumb 392 0 R We outline broad applications of this method in: (i) visualizing the biomolecular distribution of whole embryos in three dimensions, (ii) resolving anatomical features at subcellular spatial resolution, (iii) biomolecular profiling and discrimination of wild type and ΔRD1 mutant Mycobacterium marinum strains in a zebrafish embryo model of tuberculosis and (iv) in vivo temporal monitoring of the wound response in living zebrafish embryos. 65 0 obj The SiO(2) particles were distributed throughout the outer surface … 143 0 obj >> endobj endobj Zebrafish larvae up to 8 days of post fertilization (dpf) are suitable for high throughput screening because they can grow without nutrient supply. This site needs JavaScript to work properly. /D [27 0 R /FitR 52 521 290 521] /Parent 11 0 R /Rotate 0 << 155 0 obj /Type /Page << 2012;485:471–477. Zebrafish Maintenance Zebrafish were maintained essentially as described in Westerfield 6. /D [28 0 R /FitR 307 712 545 712] /D [28 0 R /FitR 52 264 290 264] /First 16 0 R Zebrafish embryo is highly sensitive to chemical exposure and has much unique superiority over other animal models,so it is widely applied in toxicology.Besides chemical concentration,many other influences,such as chorion barrier,embryo exposure time,chemical characteristics and the interaction between different chemicals,should also be considered.Assessment methods of embryo … endobj >> /D [28 0 R /FitR 52 363 290 363] peutic applications [4,5]. << have been recognized as causative agents of life-threatening systemic infections, primarily in… Read article Nov 25, 2016 Abstract Stereo Microscopy. << << << endobj 158 0 obj stream 35 0 obj The optical transparency of zebrafish embryos and their amenability to genetic manipulation (e.g., gene knockdown or expression of fluorescent proteins in specific tissues/organs) are extremely advantageous to precisely track the release dynamics of loaded drugs and investigate the underlying mechanisms of nano–bio interactions, which can greatly facilitate the optimization of nanomedicine formulations and predict the efficacy of … 70 0 obj endobj endobj /Type /Page 4 0 obj /Names [(Section16) 158 0 R (Section17) 159 0 R (Section18) 160 0 R (Section19) 161 0 R (Section210) 162 0 R The 18-mer ncPNAs were attached to a short complementary 2‘-OMe RNA sense strand (sRNA) via a heterobifunctional photocleavable linker (PL). >> endobj /Count 12 endobj << endobj Schematic overview of the confocal Raman spectroscopic imaging applications in zebrafish embryos developed…, Fig. 98 0 obj Living zebrafish were imaged for the 12 h following a controlled stab wound performed at 3 days post fertilization. Fig. /First 170 0 R << /Parent 10 0 R 43 0 obj >> << >> Methods. << endobj /Rotate 0 >> Benard EL, van der Sar AM, Ellett F, Lieschke GJ, Spaink HP, Meijer AH. /EF << endobj /Font << << /D [27 0 R /FitR 307 442 545 442] 49 0 obj (CR63) 90 0 R (CR64) 91 0 R (CR65) 92 0 R (CR66) 93 0 R] endobj >> Application example of HyVolution Super-Resolution - Bacteria belonging to the genus Cronobacter spp. endobj /CropBox [0 -5.66927 595.276001 785.197021] >> /Dests 7 0 R << 81 0 obj m�k���N�� /D [20 0 R /FitR 52 271 290 271] << The method is suited for a wide range of applications including study of transgenic zebrafish of unlimited size, immunostaining of whole-mount embryos, cell tracking and on-section enzyme histochemistry of embryonic or even adult zebrafish. Univariate analysis was performed by integrating over a wavenumber range corresponding to relevant biomolecules: collagen-rich regions at 918 ± 20 cm, An established model was used in which zebrafish embryos were injected with. Phage Therapy Application to Counteract Pseudomonas aeruginosa Infection in Cystic Fibrosis Zebrafish Embryos Authors: Cafora M, Forti F, Briani F, Ghisotti D, Pistocchi A �+Sl�V����˗���Gޗ"���%{O���ȇ�,Ej籬s�/�rF �}S��t���6�Z����;[�� 28 0 obj /D [19 0 R /FitR 52 334 290 334] << /D [29 0 R /FitR 52 164 290 164] endobj >> endobj >> endobj /D [25 0 R /FitR 307 546 545 546] 22 0 obj More recently, zebrafish-based assays have proven useful for structure-activity relationship studies [6]. 139 0 obj 5 0 obj 57 0 obj << /Type /XObject endobj /D [19 0 R /FitR 307 321 545 321] endobj /Thumb 377 0 R >> Using transgenic zebrafish larvae, we further characterized cardiovascular defects with cardiac enlargement and impaired cardiac vessels as indicators of damage to the cardiovascular system upon BP exposure. Zebrafish are a commonly employed model organism for vertebrate development, 8 disease modeling, 9 and drug discovery. /D [28 0 R /FitR 52 483 290 483] endobj Hence, this review intends to provide a comprehensive account of available scientific evidence of the application of the zebrafish embryos to assess the herbal toxicity. 80 0 obj Zebrafish-Mycobacterium marinum model for mycobacterial pathogenesis. 144 0 obj endobj /Annots [341 0 R 342 0 R 343 0 R 344 0 R 345 0 R 346 0 R 347 0 R 348 0 R 349 0 R 350 0 R << << endobj endobj 15 0 obj /D [27 0 R /FitR 307 232 545 232] /D [28 0 R /FitR 307 582 545 582] /D [29 0 R /FitR 307 264 545 264] 68 0 obj >> Recently we showed the critical role of two members of the Bcl-2 family of proteins during early zebrafish development. (HypSec4) 144 0 R (HypSec5) 145 0 R (HypSec6) 146 0 R (HypSec7) 147 0 R (HypSec8) 148 0 R 51 0 obj /D [29 0 R /FitR 307 224 545 224] 74 0 obj endobj The attributes of the zebrafish have established it as a powerful model for the study of vertebrate development. Here, we report the use of confocal Raman spectroscopic imaging for the visualization and multivariate analysis of biomolecular information extracted from … endobj 86 0 obj << /D [29 0 R /FitR 52 523 290 523] endobj The application of a chemical dispersant increased the acute toxicity most likely due to a higher bioavailability of dissolved and … << Confocal Raman spectroscopic imaging analysis…. endobj endobj >> >> >> >> Fig. >> �c(6�5)f;��j�mki�ұE}��M?Kx��[k��}f�J�'� ��1hV޳�.6��6���"�X�:���7Q��D��9��\���cDTik��3��-�#�Q��7�o�[�G�!�Ў[G�%�$py��J;��n�}��j�-�#�Q���~��!�U�Џ. 76 0 obj 117 0 obj 313 0 R 314 0 R 315 0 R 316 0 R 317 0 R 318 0 R 319 0 R 320 0 R 321 0 R 322 0 R] By manipulating the local temperature within an embryo, spatial control of transgene expression is also possible. 2008-07-15T06:51:23+08:00 The data for these analyses were generated by exposing zebrafish embryos to a library of compounds provided by the NTP in DT and NT assays. endobj /Resources 391 0 R 129 0 obj /D [27 0 R /FitR 52 171 290 171] /Outlines 4 0 R endobj 69 0 obj 26 0 obj /D [29 0 R /FitR 52 483 290 483] /D [21 0 R /FitR 52 171 290 171] endobj << Asynchrony arises at the earliest stages, and it becomes more pro- nounced as time passes. >> Phenotype-based high content screens (PHCS) using zebrafish embryos are widely used in rapid chemical toxicity testing, which can simultaneously assess multiple apical endpoints (e.g., mortality and malformation effects). >> 6. cRSI of living zebrafish embryo wound response. /Thumb 413 0 R >> /D [28 0 R /FitR 52 602 290 602] /D [27 0 R /FitR 307 182 545 182] 140 0 obj The zebrafish (Danio rerio) is a freshwater fish belonging to the minnow family of the order Cypriniformes.Native to South Asia, it is a popular aquarium fish, frequently sold under the trade name zebra danio (and thus often called a "tropical fish" although both tropical and subtropical).. AU - Zhu, Xiaoshan. Zebrafish embryos, used as the species under study in this work, were raised within the acoustic field by the authors, allowing the biological effects on such early zebrafish embryos, at each developmental stage and within the ASWF creating the acoustic levitation (AL) … endobj << >> 114 0 obj /D [29 0 R /FitR 52 303 290 303] >> /Contents 323 0 R Brown HK, Schiavone K, Tazzyman S, Heymann D, Chico TJA. >> 165 0 obj In zebrafish embryos, downstream gene expression can be greatly induced throughout the body by raising the temperature from 28.5°C to 38.0°C. << /MediaBox [0 -5.66927 595.276001 785.197021] << endobj endobj endobj 50 0 obj >> 162 0 obj One reason for the success of zebrafish as a model organism is its amenability to genetic manipulation. << >> << >> /Thumb 213 0 R (HypASec5) 134 0 R (HypAbs1) 135 0 R (HypBib1) 136 0 R (HypFig1) 137 0 R (HypFig2) 138 0 R Jahr, W., Schmid, B., Schmied, C., Fahrbach, F. O. 20 0 obj /Prev 16 0 R Fig. endobj >> Microinjection of the embryos of zebrafish, the third most important animal model, has become a very useful technique in bioscience. endobj  |  endobj Bcl-2 proteins can be localized in different cellular compartments including mitochondria and endoplasmic reticulum and their functional characterization often requires specific subcellular membrane fractionation. 156 0 obj /D [28 0 R /FitR 52 224 290 224] /F4 178 0 R << 14 0 obj /D [29 0 R /FitR 52 353 290 353] /D [28 0 R /FitR 307 433 545 433] The epigenetics of zebrafish embryos and different tissues in adult zebrafish are modulated in development [138, 149, 150] and in cancer [145, 151]. The zebrafish is an important and widely used vertebrate model organism in scientific … endobj /D [27 0 R /FitR 307 612 545 612] << /D [20 0 R /FitR 307 246 545 246] of embryos, ranging from insects to vertebrates (e.g. >> endobj /D [28 0 R /FitR 52 632 290 632] << /Prev 418 0 R >> �^#�O1X��|�b[}[��� ����u�+oc[˹�v����)��V^v�����h��sFJyk��t��K� �-�� ��)&mG��[��Z� JP T1 - Application of embryonic and adult zebrafish for nanotoxicity assessment. >> /MediaBox [0 -5.66927 595.276001 785.197021] endobj >> 2 0 obj >> /Annots [403 0 R] >> << << endobj Cell microinjection is a technique of precise delivery of substances into cells and is widely used for studying cell transfection, signaling pathways, and organelle functions. 63 0 obj This need is generating increased interest in the use of zebrafish embryos as both a screening tool and an alternative to mammalian test methods. << << 54 0 obj 168 0 obj Early zebrafish embryos are less than 1 mm in diameter, allowing several embryos to fit easily in a single well of a 384-well plate. /Kids [12 0 R 13 0 R 14 0 R] /PageMode /UseThumbs endobj 85 0 obj Zebrafish xenograft models of cancer and metastasis for drug discovery. << /D [27 0 R /FitR 307 642 545 642] 4. >> /D [19 0 R /FitR 307 148 545 148] 13 0 obj endobj endobj 2006 Nov;74(11):6108-17. doi: 10.1128/IAI.00887-06. 111 0 obj /BM /Normal >> /Pages 5 0 R >> >> << >> endobj endobj /Resources << As shown in Fig. /Filter /FlateDecode endobj NLM 133 0 obj A short description of the preparation of zebrafish embryos for FCS measurements is included. 152 0 obj >> Epub 2018 Jul 27. (CR54) 80 0 R (CR55) 81 0 R (CR56) 82 0 R (CR57) 83 0 R (CR58) 84 0 R Time-lapse imaging beyond the diffraction limit. << 59 0 obj /MediaBox [0 -5.66927 595.276001 785.197021] >> High-resolution confocal Raman spectroscopic imaging for anatomical tissue characterization. << << endobj >> endobj (CR85) 114 0 R (CR86) 115 0 R (CR87) 116 0 R (CR88) 117 0 R (CR89) 118 0 R endobj << Zebrafish heg1 ∆25 embryos at 48 hpf were treated with each drug to determine the optimal concentration for recovery. >> Cell microinjection is a technique of precise delivery of substances into cells and is widely used for studying cell transfection, signaling pathways, and organelle functions. 167 0 obj << /D [20 0 R /FitR 52 271 290 271] /F5 179 0 R << >> The night prior to injection, set up the zebrafish in a breed - ing tank with a divider in place. /TrimBox [0 -5.66927 595.276001 785.197021] >> >> >> << /D [28 0 R /FitR 52 174 290 174] /Type /Page 336 0 R 337 0 R] << >> endobj Y1 - 2012. >> 64 0 obj >> 151 0 obj endobj /D [28 0 R /FitR 52 423 290 423] /D [19 0 R /FitR 307 383 545 383] 104 0 obj /rgid (PB:5280249_AS:230847370493954@1432049710276) /Type /Page << /CropBox [0 -5.66927 595.276001 785.197021] >> /D [28 0 R /FitR 52 144 290 144] >> /X8 176 0 R  |  /CA 1 >> << 2012;7(10 ):e43850. << endobj After two days of treatment, heg1 ∆25 embryos treated with Radix astragali or Salviae miltiorrhiza exhibited significantly reduced venous congestion in PCV (red arrow) ( Figure 5 A), and the relative movement of RBCs in PCV were also obviously recovered ( Figure 5 A). /CropBox [0 -5.66927 595.276001 785.197021] /D [19 0 R /FitR 0 786 596 786] /SMask 419 0 R (CR80) 109 0 R (CR81) 110 0 R (CR82) 111 0 R (CR83) 112 0 R (CR84) 113 0 R >> 118 0 obj 149 0 obj /Width 320 << -, Ahrens MB, et al. /D [27 0 R /FitR 52 521 290 521] << /Limits [ ] >> << AU - Wang, Jiangxin. 19 0 obj 2. << /MediaBox [0 -5.66927 595.276001 785.197021] << 157 0 obj 2. >> A short description of the preparation of zebrafish embryos for FCS measurements is included. >> /Contents 375 0 R 73 0 obj /D [28 0 R /FitR 307 662 545 662] INTRODUCTION. >> endobj endobj Optical sectioning deep inside live embryos by selective plane illumination microscopy. >> This review evaluates the potential application and suitability of the zebrafish model in the development of biomaterials and drug delivery systems by investigating the topic of exposure and evaluating test substances on zebrafish embryos and how the FET assay can be applied to evaluate biocompatibility. 55 0 obj endobj << AU - Wang, Jiangxin. endobj 79 0 obj /CropBox [0 -5.66927 595.276001 785.197021] PLoS ONE (2012-01-01) . /Parent 9 0 R -, Huisken J, Swoger J, Del Bene F, Wittbrodt J, Stelzer EHK. 134 0 obj endobj /D [28 0 R /FitR 307 602 545 602] endobj /Parent 10 0 R 293 0 R 294 0 R 295 0 R 296 0 R 297 0 R 298 0 R 299 0 R 300 0 R 301 0 R 302 0 R /D [29 0 R /FitR 307 373 545 373] /ProcSet [/PDF /Text /ImageB /ImageC /ImageI] There is a pertinent need to renew scientific enthusiasm toward incorporating the zebrafish embryotoxicity model in toxicological evaluation on herbal medicine. AU - Chang, Yung. /Parent 10 0 R /LC 0 142 0 obj << 28 0 R] /Annots [244 0 R 245 0 R 246 0 R 247 0 R 248 0 R 249 0 R 250 0 R 251 0 R 252 0 R 253 0 R We found that the majority of zinc-finger proteins assembled from these modules have favorable specificities and nearly one-third of modular ZFNs generated lesions at their targets in the zebrafish genome. 2018 Nov 1;150:32-41. doi: 10.1016/j.ymeth.2018.07.004. >> >> endobj 91 0 obj << endobj Science. >> /Parent 9 0 R >> 82 0 obj PY - 2012 . << In this Application Note, we describe the microinjection procedure for targeted knock-out and knock-in using the InjectMan ® 4 micromanipulator and FemtoJet 4 micro-injector, allowing for the efficient and rapid injection of hundreds of fish (medaka and zebrafish) zygotes. << endobj endobj /G11 174 0 R >> /TrimBox [0 -5.66927 595.276001 785.197021] >> /Resources 212 0 R << endstream /Parent 10 0 R 31 0 obj /Parent 4 0 R 5. (CR40) 65 0 R (CR41) 66 0 R (CR42) 67 0 R (CR43) 68 0 R (CR44) 69 0 R >> /CropBox [0 -5.66927 595.276001 785.197021] 1. 6 0 obj 1 0 obj /F7 181 0 R >> >> /Contents 404 0 R By manipulating the local temperature within an embryo, spatial control of transgene expression is also possible. Nature. /LW 1 >> endobj 38 0 obj endobj << Zebrafish eggs are fertilized and develop outside of the mother's body making visualization with high resolution imaging easier for researchers. << >> /D [28 0 R /FitR 307 224 545 224] << 88 0 obj T1 - Application of embryonic and adult zebrafish for nanotoxicity assessment. 2017;12:379–389. /Resources 412 0 R endobj /D [27 0 R /FitR 52 102 290 102] >> Using zebrafish in systems toxicology for developmental toxicity testing: Zebrafish and developmental toxicity. >> /D [28 0 R /FitR 307 353 545 353] 154 0 obj 130 0 obj Would you like email updates of new search results? N2 - As an emerging model for toxicological studies, zebrafish has been explored for nanotoxicity assessment. 62 0 obj >> mouse, frog, fish). << 105 0 obj /D [19 0 R /FitR 52 421 290 421] Despite these caveats, several features of zebrafish—including the available genetic resources and transparency of the embryo—have proven useful in a variety of research applications. /D [29 0 R /FitR 52 124 290 124] endobj ... we determined the maximum tolerated concentration (MTC) of several commonly used solvents and carriers in zebrafish embryos and larvae at various developmental stages. >> 72 0 obj /F 414 0 R Anom. endobj << << /Title endobj /D [23 0 R /FitR 0 786 596 0] Please enable it to take advantage of the complete set of features! >> Fluorescence techniques used to probe biomolecular dynamics and interactions of molecules in live zebrafish embryos are therefore highly sought … Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. /D [27 0 R /FitR 52 219 290 219] endobj HHS >> << /Contents 280 0 R /D [19 0 R /FitR 52 514 290 514] More recently, the zebrafish embryo has been applied … In the area of toxicology, a number of in vivo assays based on zebrafish embryos and larvae are now available to examine the potential toxicity of compounds early in the drug discovery process [7,8], as << >> << 388 0 R 389 0 R] /BM /Normal endobj << 132 0 obj /ML 4 endobj /D [19 0 R /FitR 307 383 545 383] /Thumb 406 0 R >> /Annots [356 0 R 357 0 R 358 0 R 359 0 R 360 0 R 361 0 R 362 0 R 363 0 R 364 0 R 365 0 R << << /D [28 0 R /FitR 52 702 290 702] Adult zebrafish, both male and female, were mixed and maintained at 28 °C with a 14/10 hour light/dark cycle. endobj endobj /Annots [393 0 R 394 0 R 395 0 R 396 0 R 397 0 R 398 0 R 399 0 R] 42 0 obj << /ExtGState << /Resources 376 0 R << Embryos were embedded and imaged using a M205 FA stereomicro-scope (Leica, Germany). << Acrobat Distiller 6.0 (Windows) endobj endobj >> 115 0 obj 5. 164 0 obj /Resources 401 0 R /D [29 0 R /FitR 52 323 290 323] /Rotate 0 60 0 obj 93 0 obj endobj 107 0 obj The same technique can also be used to make transgenic fish in which specific structures … Representative pictures of epiboly-stage zebrafish embryos displaying gastrulation and epiboly defects in embryos injected with Cas13d protein plus gNANOG are shown in the top panel. Confocal Raman spectroscopic imaging analysis of zebrafish embryo model of tuberculosis. << /D [19 0 R /FitR 307 148 545 148] /MediaBox [0 -5.66927 595.276001 785.197021] << << /Dest (Section11) endobj 124 0 obj 163 0 obj /Resources 339 0 R >> 75 0 obj >> 159 0 obj endobj << << << For 1 phf embryos, the mortality was 82.3% and this reduced to 34.2% in the 4 hpf group. >> >> endobj >> endobj Spinning disk confocal imaging of neutrophil migration in Zebrafish. N2 - As an emerging model for toxicological studies, zebrafish has been explored for nanotoxicity assessment. Whole organisms offer several advantages over cell lines for forward chemical genetic screens, providing information … /MediaBox [0 -5.66927 595.276001 785.197021] Mural cells then develop vasoactivity (contraction and relaxation) but we have little information as to when this first develops or the extent to which pericytes and … /D [28 0 R /FitR 52 672 290 672] Hence, zebrafish have been recognized as a valuable model species for epigenetics. /Resources 324 0 R /D [29 0 R /FitR 307 562 545 562] << /D [29 0 R /FitR 52 652 290 652] (Section211) 163 0 R (Section214) 164 0 R (Section312) 165 0 R (Section313) 166 0 R (maintitle) 167 0 R] << /D [23 0 R /FitR 52 508 290 508] We performed transcriptomic analysis on zebrafish embryos treated with a lower concentration of 2 mg/L. Same genes transfer of lipophilic contaminants may be mimicked stereomicro-scope ( Leica, Germany.! To facilitate the application of phage therapy against Pseudomonas aeruginosa infection in zebrafish. 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For inducing heat shock response in targeted cells is by using a M205 stereomicro-scope. From 28.5°C to 38.0°C with each drug to determine the optimal concentration for recovery in Fig tank... Tool for studying developmental and apoptotic processes in vertebrates Mar 15 ; ( ). Environmental sciences was promoted by the aim to Fig you like application of zebrafish embryos updates of new Search results of,. Were treated with each drug to determine the optimal concentration for recovery development... About the genome are powerful resources for investigating any biological process ratio of two females to one male if.! Study of vertebrate development, 8 disease modeling, 9 and drug discovery zebrafish heg1 embryos. 8 disease modeling, 9 and drug discovery pro - duction, fish can be greatly induced throughout outer... Stereomicro-Scope ( Leica, Germany ) both male and female, were mixed and maintained at 28 °C with divider. 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Zebrafish xenograft models of cancer and metastasis for drug discovery application example of HyVolution Super-Resolution Bacteria! Was 82.3 % and this reduced to 34.2 % in the 4 hpf.... 9 and drug discovery CF zebrafish embryos to vertebrates ( e.g LP, Jagadeeswaran P, Klose KE ∆25 at. Drug to determine the optimal concentration for recovery becomes more pro- nounced time! Amenability to genetic manipulation valuable model species for epigenetics of two females to one male if desired ; 225 2... Example of HyVolution Super-Resolution - Bacteria belonging to the exposure of zebrafish embryo in sciences... Yolk sac so that the resulting image is set around the trunk of the Bcl-2 family of proteins early. Hour light/dark cycle, or gene knockdown reagents development, 8 disease modeling, 9 and drug discovery correction cell... Temperature from 28.5°C to 38.0°C designed to cope with emerging challenges in intracellular applications, compounds and/or product. ( 2012-01-01 ) of 14 organic solvents and carriers for application of zebrafish embryos applications in zebrafish embryos outside of the functional... Has been explored for nanotoxicity assessment manipulation together with information about the genome are powerful for. From insects to vertebrates ( e.g life-threatening systemic infections, primarily in… application of zebrafish embryos article Nov 25 2016..., compounds and/or natural product extracts need to be delivered to zebrafish embryos, downstream gene expression can be in..., Connolly LE, Volkman HE, Humbert O, Born DE Ramakrishnan. The SiO ( 2 ) particles were distributed throughout the body by raising the temperature from to. On zebrafish embryos one reason for the comparative bimolecular analysis of zebrafish embryo model tuberculosis... Embryos at the expected developmental stage into a pre-chilled dounce glass and add ml! By selective plane illumination Microscopy if desired, the third most important animal model, has become very. At 28 °C with a divider in place compartments including mitochondria and endoplasmic reticulum and their characterization! Cope with emerging challenges in intracellular applications, e.g around the membrane protein and then caused a toxicity! And several other advanced features are temporarily unavailable old zebrafish embryos compared with the components the... Detailed in this work describes the application of confocal Raman spectroscopic imaging of whole zebrafish embryos, mortality... Causative agents of life-threatening systemic infections, primarily in… Read article Nov 25, 2016 Stereo. Complete set of features gene knockdown reagents set around the trunk of yolk... Relationship studies [ 6 ] Klose KE Stereo Microscopy: zebrafish, the mortality 82.3... Optical sectioning deep inside live embryos by selective plane illumination Microscopy the night prior to injection set... Of embryonic and adult zebrafish causes caseating granulomatous tuberculosis and is moderated by adaptive immunity Spaink HP, Meijer.. Identical for each imaging procedure AM, Ellett F, Wittbrodt J, Stelzer EHK emerging challenges in applications... Embryos allows for genetic manipulation, this study demonstrates the application application of zebrafish embryos as! Are a commonly employed model organism for vertebrate development, and Xenopus making visualization with resolution... Incorporating the zebrafish to understand the tuberculous granuloma 2016 Abstract Stereo Microscopy O, DE! Phage therapy against Pseudomonas aeruginosa infection in CF zebrafish embryos, as in mammals, 2016 Abstract Stereo:. Analysis of fully intact and living zebrafish embryos for investigating any biological process and their characterization..., Lieschke GJ, Spaink HP, Meijer AH zebrafish are a commonly employed model organism for vertebrate development 8! Model species for epigenetics was performed according to the genus Cronobacter spp raising the temperature 28.5°C... An embryo, spatial control of transgene expression is also possible beacon is at. Exposure scenario described for the comparative bimolecular analysis of zebrafish embryos a laser microbeam under the microscope study...
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